Main Content

Xtract™ DNA Kit

For Dried Blood Spot

The Spot On Sciences DNA extraction Kit is an efficient and cost effective method that simplifies the extraction and isolation of genomic DNA from a dried matrix. Isolation is achieved in less than two hours without phenol/chloroform extractions or the hassle of a precipitated pellet. 10-20 ng of PCR-ready Genomic DNA can be isolated from every microliter of blood applied to the HemaSpot -HF device (100-200 ng of genomic DNA per blade). No other preparation or purification is needed.

* For Research Use Only. Not For Use in Diagnostic Procedures.

Highlights

  • PCR-ready genomic DNA isolated from dried blood spots in less than 2 hours
  • Optimized for DNA extraction from the  HemaSpot -HF blood collection device
  • Simple protocol
    • No phenol chloroform extractions
    • No columns
    • No pellet
  • Efficient extraction
    • Yields 6 - 20 ng of genomic DNA per every µl of blood applied to sample
  • Cost effective
Kit Contents:Component
Isolations100 preps
10X Buffer A8 mL
Buffer B12 mL
10X TE Buffer12 mL

 

Results

Figure 1. GAPDH QPCR from genomic DNA template: 1-4 µl of DNA isolated from HemaSpot -HF dried blood spots by the Xtract DNA kit was used as template for Taqman® RT-PCR. The linear curves demonstrate the lack of inhibition of QPCR products by the DNA template.
Figure 2. DNA recovery from eight individual blades of the
HemaSpot -HF device.

Products

Xtract DNA Kit

Xtract DNA Extraction Kit

The DNA extraction Kit is an efficient and cost effective method that simplifies the extraction and isolation of genomic DNA from a dried matrix.

Not available for online purchase. Request quote below.

Request Quote

This product is not yet available for online purchase. Please fill out the form below to request a quote.