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Xtract™ DNA Kit

For Dried Blood Spot

The Spot On Sciences DNA extraction Kit is an efficient and cost effective method that simplifies the extraction and isolation of genomic DNA from a dried matrix. Isolation is achieved in less than two hours without phenol/chloroform extractions or the hassle of a precipitated pellet. 10-20 ng of PCR-ready Genomic DNA can be isolated from every microliter of blood applied to the HemaSpot -HF device (100-200 ng of genomic DNA per blade). No other preparation or purification is needed.

* For Life Science Research Use Only. Not For Use in Diagnostic Procedures.


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Xtract DNA Kit

Highlights

  • PCR-ready genomic DNA isolated from dried blood spots in less than 2 hours
  • Optimized for DNA extraction from the  HemaSpot -HF blood collection device
  • Simple protocol
    • No phenol chloroform extractions
    • No columns
    • No pellet
  • Efficient extraction
    • Yields 6 – 20 ng of genomic DNA per every µl of blood applied to sample
  • Cost effective
Kit Contents: Component
Isolations 100 preps
10X Buffer A 8 mL
Buffer B 12 mL
10X TE Buffer 12 mL

Results

Figure 1. GAPDH QPCR from genomic DNA template: 1-4 µl of DNA isolated from HemaSpot -HF dried blood spots by the Xtract DNA kit was used as template for Taqman® RT-PCR. The linear curves demonstrate the lack of inhibition of QPCR products by the DNA template.
Figure 2. DNA recovery from eight individual blades of the
HemaSpot -HF device.

Downloads


Xtract DNA Kit One Sheet


Xtract DNA Kit Instructions For Use

Products

Xtract DNA Kit

Xtract DNA Extraction Kit

The DNA extraction Kit is an efficient and cost effective method that simplifies the extraction and isolation of genomic DNA from a dried matrix.

Not available for online purchase. Request quote below.

Request Quote

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